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Assessment of markers expressed in human hair follicles according to different skin regions


Background: Body region-dependent hair follicle (HF) traits are involved with follicular measurement and distribution, and have been demonstrated to have traits for every area of the physique.

Objectives: The intention of the current examine was to analyze the expression patterns of the markers known as cytokeratin 15 (K15), cytokeratin 6 (K6) and monoclonal antibody Ki-67, and likewise apoptosis in HFs, which could be noticed in numerous components of the human physique.

Material and strategies: In this examine, wholesome human HFs had been taken by biopsy from 5 varied donor websites of the human physique: the scalp, the leg, the stomach, the again and waist. HF-containing pores and skin specimens taken utilizing cryosection had been stained with hematoxylin & eosin (H&E) and K15, K6, Ki-67 and terminal deoxynucleotidyl transferase-mediated digoxigenin-dNTP nick end-labelling (TUNEL) immunofluorescence staining protocol was carried out.

Results: Different pores and skin areas from the human physique had been examined histologically. While the HFs of scalp tissue confirmed anatomically apparent hair layers, some hair sections from different areas, just like the leg, the stomach, again and waist, weren’t as distinct as within the scalp area. According to our findings, K15 expression was highest within the scalp. In addition, the immunoreactivity (IR) depth of K15 was considerably decreased within the HFs on the waist and belly areas, in comparison with the scalp and again areas (p < 0.001). However, the IR depth of K6 within the scalp area was statistically considerably larger than the IR depth of K6 within the stomach area (p < 0.05). Moreover, we confirmed intraepithelial apoptosis and proliferation of keratinocytes within the bulge of HF. In the examine, Ki-67-positive and TUNEL-positive cell numbers weren’t statistically vital (p > 0.05).

Conclusions: Our findings are essential for additional investigation of molecular facets of the human hair follicle stem cells compartments in well being and illness, which may be a promising mannequin for comparative research with completely different human ailments.

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